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KMID : 1123920080220020397
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Korean Journal of Oriental Physiology and Pathology
2008 Volume.22 No. 2 p.397 ~ p.402
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Preparation of Black Panax ginseng Leaf and Evaluation of its Antioxidative Effect
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Kim Sang-Kyum
Kim Eui-Keom
Lee Jee-Hyun
Cho Soo-Hyun
Shen Gui-nan
Kim Long-Guo
Oh Jung-Min
Myung Chang-Seon
Oh Han-Jin
Kim Dong-Hee
Yun Mi-Young
Yun Jae-Don
Roh Seong-Soo
Park Yong-Jin
Seo Young-Bae
Song Gyu-Yong
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Abstract
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The purpose of this study is to prepare black Panax ginseng leaf (PGL) and evaluate its antioxidative effect. In order to make black PGL, the raw PGL was successiely steamed at for 3 hr nine times. The antioxidant activities of total saponins (Sa) from PGL and black PGL against peroxyl radicals and peroxynitrites were determined by the total oxy-radical scavenging capacity (TOSC) assay. Specific TOSC values for black PGL-Sa against peroxyl radicals and peroxynitrites were 2.3-fold and 2.1-fold of PGL-Sa, respectively, and 2.2-fold and 5.2-fold of glutathione, a positive control antioxidant, respectively. The black PGL-Sa exhibited stronger antioxidative effect than PGL-Sa. The main ginsenosides of black PGL were . Among the saponins in black PGL, the amount of ginsenoside was examined by HPLC. 22.12 mg of ginsenoside was obtained from 1g of dried black PGL.
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KEYWORD
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Panax ginseng leaf, Black Panax ginseng leaf, Antioxidative effect, TOSC assay, Ginsenoside
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